Shoot induction and plant regeneration from receptacle tissues of Lilium longi ̄orum
نویسندگان
چکیده
An ef®cient system has been developed for the in vitro plant regeneration of Lilium longi ̄orum Thunb. by culturing receptacle sections from ̄ower buds. The sections were cultured on one-half MS medium plus 30 g lÿ1 sucrose, 8 g lÿ1 agar, 5.4 mM NAA or 4.9 mM IBA plus 2.2 mM BAP. A section size of 3±4 mm was found to be optimal. After 60 days an average of 41 shoots were formed per explant. More vigorous shoots were obtained by subculturing on hormone-free medium with 20 g lÿ1 sucrose. Rooting occurred on one-half MS medium with 1.1 mM NAA. Rooted plants were hardened-off in a greenhouse for two months, and normal ̄owering plants were produced. # 2001 Elsevier Science B.V. All rights reserved.
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